Translational_Unit

Part:BBa_K3288055

Designed by: Jason Park   Group: iGEM19_SIS_Korea   (2019-10-16)


light inducible expression of signal peptide (LamB) fused PETase(28-290;WT)

BBa_K3288055 is a composite part in which BBa_K1075044, BBa_K3288007 and BBa_K3288010 are combined.

This part is compatible with RFC10.

Usage and Biology

SIS_Korea_figure5.jpeg

We introduced a light inducible system to conveniently and effectively overexpress an enzyme that degrades PET without using chemical inducers.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2171
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 63
    Illegal NgoMIV site found at 195
    Illegal NgoMIV site found at 289
    Illegal NgoMIV site found at 582
    Illegal NgoMIV site found at 1076
    Illegal NgoMIV site found at 1094
    Illegal NgoMIV site found at 1184
    Illegal AgeI site found at 414
    Illegal AgeI site found at 1542
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1643
    Illegal BsaI.rc site found at 525


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Categories
Parameters
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